Basic statistics in Prism

E-Learning

basic statistics in prism

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Please register on the training website to access this e-learning course. There are no online or face-to-face sessions.

Data sets you need in this course.

Questions can be asked by contacting janick.mathys@vib.be

Check our Basic statistics theory course on this elearning system for the statistical background.

Course materials

Graphpad tutorials:

- Prism user guide: how to use Prism

- Prism statistics guide: the theoretical background of the statistical analyses in Prism

- Prism curve fitting guide: how to do regression in Prism

- Prism video tutorials

Course Content

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The Prism user interface 2 Topics | 1 Quiz

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Prism user interface

Table types in Prism

Simple statistical tests in Prism 18 Topics | 9 Quizzes

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Getting the data into Prism

How to stack the values?

Checking normality

How to check normality?

Unpaired t test

How to interpret the p-values of the tests

Report the outcome of the unpaired t test

How to graph the outcome of the unpaired t test

Graph the results of the unpaired t test

Create a new table

Use the first row as column names

Normally distributed?

Normally distributed?

Make a histogram

Report and graph the outcome of a Mann Whitney test

Compare 2 groups of paired data

Compare dependent data

Report the outcome of a paired t test

Graph the results of a paired t test

Comparing ratios

Compare more than 2 groups

Compare more than 2 groups

Report the outcome of a one-way ANOVA

Report the outcome of a Kruskal Wallis test

Nested one-way ANOVA

Advanced statistical tests in Prism 8 Topics | 5 Quizzes

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A data set with two factors

Import 2 factor data

Compare groups defined by two factors

Limitations of two-way ANOVA in Prism

Finding DE genes / proteins / metabolites

What do you want to know?

Only use Prism to analyze small scale omics data

Import the data into Prism

Multiple tests, one per row in Prism

Are metabolites affected by treatment?

Volcano plot and heat map

Three-way ANOVA

Make a subset of a table

Graphs in Prism 5 Topics

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The user interface for graphs

Set the characteristics of a graph: color scheme, size

Create a layout

Make graphs consistent

Check the relation between variables 11 Topics | 8 Quizzes

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Import data for linear regression

Import data for linear regression

Linear regression – compare slopes

Linear regression

Results of the linear regression

Format of data for a standard curve

Import data for standard curve

Interpolation from a standard curve

Creating a standard curve

Add Interpolated values to the plot

Nonlinear regression: dose-response data

Data for dose-response curve

Compare dose-response curves

Are the two curves different?

Data for correlation analysis

Data for correlation analysis

Correlation (with a user-defined transformation)

Correlation matrix and correlogram

Compare groups of categorical data 2 Topics | 1 Quiz

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Import data for chi square test

Table type for chi square test

Chi square test

Survival analysis in Prism 2 Topics | 1 Quiz

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Survival analysis

Survival analysis

Create the Kaplan Maier plot

Contributors: Janick Mathys

Created by Janick Mathys

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