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When you do paired end sequencing how many files are generated per sample? Answer should be an integer.
In paired end sequencing reads from the same fragment should overlap.
What will be the required steps in between the two rounds of sequencing? Multiple answers are correct.
When you do paired end sequencing you get 2 files per sample:
But the reads from the 2 files form pairs because they come from the same fragment. How can you find the reads that form a pair (i.e. that come from the same fragment in the library)?
During alignment what is the maximum distance between reads that form a pair?